TLR7 Antibody

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NSJ Bioreagents
Product Code: NSJ-R32339
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32339-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TLR7 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 7
Western blot testing of 1) MCF7, 2) COLO320 and 3) Jurkat lysate with TLR7 antibody. Expected/observed molecular weight ~121 kDa.
2 / 7
ICC testing of A549 cells with TLR7 antibody.
3 / 7
IHC testing of FFPE human placenta with TLR7 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
4 / 7
IHC testing of frozen human placenta with TLR7 antibody.
5 / 7
IHC testing of FFPE mouse thymus with TLR7 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
6 / 7
IHC testing of FFPE rat thymus with TLR7 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
7 / 7
Flow cytometry testing of human PBMC with TLR7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TLR7 antibody.
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Western blot testing of 1) MCF7, 2) COLO320 and 3) Jurkat lysate with TLR7 antibody. Expected/observed molecular weight ~121 kDa.
ICC testing of A549 cells with TLR7 antibody.
IHC testing of FFPE human placenta with TLR7 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placenta with TLR7 antibody.
IHC testing of FFPE mouse thymus with TLR7 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of FFPE rat thymus with TLR7 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Flow cytometry testing of human PBMC with TLR7 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TLR7 antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the TLR7 antibody should be determined by the researcher.
Description:
TLR7 (Toll-like receptor 7) is protein that in humans is encoded by the TLR7 gene. Orthologs are found in mammals and birds. TLR7 is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on human chromosome X. TLR7 recognises single stranded RNA in endosomes, which is a common feature of viral genomes which are internalised by macrophages.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids QAHPYFWQCLKNALATDNHVAYSQVFKE of human TLR7 were used as the immunogen for the TLR7 antibody.
Limitation:
This TLR7 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9NYK1