TRAF4 Antibody

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NSJ Bioreagents
Product Code: NSJ-R31116
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31116-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TRAF4 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot testing of TRAF4 antbody; Lane 1: rat thymus; 2: human HeLa; 3: (h) Jurkat; 4: mouse HEPA cell lysate. Predicted molecular weight: ~54 kDa.
2 / 3
IHC-P: TRAF4 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 3
IHC-P: TRAF4 antibody testing of rat lymphonodus tissue. HIER: steamed with pH6 citrate buffer.
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Western blot testing of TRAF4 antbody; Lane 1: rat thymus; 2: human HeLa; 3: (h) Jurkat; 4: mouse HEPA cell lysate. Predicted molecular weight: ~54 kDa.
IHC-P: TRAF4 antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
IHC-P: TRAF4 antibody testing of rat lymphonodus tissue. HIER: steamed with pH6 citrate buffer.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the TRAF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TNF Receptor-Associated Factor 4, also called CART1 or MLN62, is a protein that in humans is encoded by the TRAF4 gene. It is a member of the TNF receptor associated factor(TRAF) family, a family of scaffold proteins. Tomasetto et al.(1995) identified genes that are overexpressed in breast cancer by differential screening of cDNAs from breast cancer-derived metastatic axillary lymph nodes. The TRAF4 cDNA, which they designated MLN62, was mapped to 17q11-q12 by radioisotopic in situ hybridization. To investigate the biologic function of TRAF4, Regnier et al.(2002) generated Traf4-deficient mice by gene disruption.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human TNF Receptor-Associated Factor 4 (PKFISHQDIRKRNYVRDD) was used as the immunogen for this TRAF4 antibody (100% homologous in human, mouse and rat).
Limitation:
This TRAF4 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9BUZ4