TRAM Antibody

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NSJ Bioreagents
Product Code: NSJ-R31117
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31117-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TRAM antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of TRAM antbody; Lane 1: rat brain; 2: (r) kidney; 3: human 293T; 4: (h) Raji; 5: (h) Jurkat cell lysate. Expected/observed size ~43KD
2 / 4
IHC-P: TRAM antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
3 / 4
ICC testing of TRAM antibody and HeLa cells
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IHC-P: TRAM antibody testing of rat kidney tissue. HIER: steamed with pH6 citrate buffer.
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Western blot testing of TRAM antbody; Lane 1: rat brain; 2: (r) kidney; 3: human 293T; 4: (h) Raji; 5: (h) Jurkat cell lysate. Expected/observed size ~43KD
IHC-P: TRAM antibody testing of human lung cancer tissue. HIER: steamed with pH6 citrate buffer.
ICC testing of TRAM antibody and HeLa cells
IHC-P: TRAM antibody testing of rat kidney tissue. HIER: steamed with pH6 citrate buffer.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the TRAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
By crosslinking and reconstitution of canine proteoliposomes, followed by microsequencing and PCR screening of canine kidney and HeLa cell cDNA libraries, Gorlich et al.(1992) isolated cDNAs encoding TRAM (translocating chain-associating membrane protein). The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 8. Sequence analysis predicted that human TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. Functional analysis indicated that the protein influences glycosylation and is stimulatory or required for the translocation of secretory proteins.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human Translocating chain-associating membrane protein 1 (KFINFQLRRWREHSAFQA) was used as the immunogen for this TRAM antibody (100% homologous in human, mouse and rat).
Limitation:
This TRAM antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q15629