Trem1 Antibody

Western Blot: 0.5-1ug/ml,ELISA (Capture; recombinant mouse protein): 0.1-0.5ug/ml (BSA-free format available)

Optimal dilution of the Trem1 antibody should be determined by the researcher.

Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide

Trem1, Triggering receptor expressed on myeloid cells-1, is encoded by TREM1 gene. The expression of Trem1 is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. Trem1 is a 30-kD glycoprotein that is reduced to 26 kD by deglycosylation, in agreement with the predicted molecular mass. The Trem1 gene which contains 4 exons maps to chromosome 6p21.1, within a TREM gene cluster and the mouse Trem1 gene maps to chromosome 17 in a region that shows homology of synteny to human chromosome 6. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide (LPS), gram-negative bacteria, and fungi. Cross-linking of Trem1 on neutrophils induces interleukin-8 (IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1 (MCP1, or SCYA2) and tumor necrosis factor (TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Trem1 engagement also induces upregulation of adhesion molecules (e.g., ITGB1) and costimulatory molecules (e.g., CD40). Trem1 is associated with DAP12 (TYROBP), a molecule frequently associated with activating receptors.

Antigen affinity purified

0.5mg/ml if reconstituted with 0.2ml sterile DI water

A recombinant mouse protein corresponding to amino acids A21-S202 was used as the immunogen for the Trem1 antibody.

This Trem1 antibody is available for research use only.

Antigen affinity

Mouse, Rat

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