Trem1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30663
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30663-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Mouse
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Trem1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of Trem1 antibody and Lane 1: recombinant mouse protein 10ng; 2: 5ng; 3: 2.5ng. The chimeric recombinant protein used for this test contains AA 21-202 + human Fc and is glycosylated.
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IHC-P: Trem1 antibody testing of mouse spleen tissue
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Western blot testing of recombinant mouse Trem1 protein (1ng/lane).
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Western blot testing of 1) rat liver, 2) mouse liver and 3) mouse spleen lysate with Trem1 antibody. Predicted molecular weight ~26 kDa.
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Western blot testing of Trem1 antibody and Lane 1: recombinant mouse protein 10ng; 2: 5ng; 3: 2.5ng. The chimeric recombinant protein used for this test contains AA 21-202 + human Fc and is glycosylated.
IHC-P: Trem1 antibody testing of mouse spleen tissue
Western blot testing of recombinant mouse Trem1 protein (1ng/lane).
Western blot testing of 1) rat liver, 2) mouse liver and 3) mouse spleen lysate with Trem1 antibody. Predicted molecular weight ~26 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the Trem1 antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. The chimeric recombinant protein used for testing contains AA 21-202 + human Fc and is glycosylated.
Description:
Triggering receptor expressed on myeloid cells-1, is encoded by TREM1 gene. Expression is seen is in monocytes and neutrophils but not in lymphocytes, dendritic cells, or other cell types. The expression of Trem1 is upregulated by stimulation with lipopolysaccharide(LPS), gram-negative bacteria, and fungi. Cross-linking of TREM1 on neutrophils induces interleukin-8(IL8) and myeloperoxidase secretion, while cross-linking on monocytes induces not only secretion of IL8 but also of monocyte chemotactic protein-1(MCP1, or SCYA2) and tumor necrosis factor(TNF); MCP1 and TNF secretion could be further upregulated by LPS-mediated priming. Engagement also induces upregulation of adhesion molecules(e.g., ITGB1) and costimulatory molecules(e.g., CD40). TREM1 is associated with DAP12(TYROBP), a molecule frequently associated with activating receptors.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 74-90 (FTRPSEVHMGKFTLKHD-mouse) were used as the immunogen for this Trem1 antibody.
Limitation:
This Trem1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Mouse, Rat
Uniprot #:
Q9JKE2