TRIF Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R31002
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31002-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TRIF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 3
Western blot with TRIF antibody and Lane 1: Jurkat; 2: HL-60 cell lysate
2 / 3
IHC-P: TRIF antibody testing of human breast cancer tissue
3 / 3
Western blot with TRIF antibody and recombinant human protein; Lane 1: 5ng; 2: 2.5ng; 3: 1.25ng
❮❯

Western blot with TRIF antibody and Lane 1: Jurkat; 2: HL-60 cell lysate
IHC-P: TRIF antibody testing of human breast cancer tissue
Western blot with TRIF antibody and recombinant human protein; Lane 1: 5ng; 2: 2.5ng; 3: 1.25ng

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the TRIF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
TIR Domain-containing adaptor molecule 1, also known as TICAM1 or TRIF, is an adapter in responding to activation of toll-like receptors (TLRs). It mediates the delayed cascade of two TLR-associated signaling cascades, where the other one is dependent upon a MyD88 adapter. By genomic sequence analysis, Oshiumi et al.(2003) mapped the gene to chromosome 19p13.3. By coimmunoprecipitation analysis, Oshiumi et al.(2003) showed that the protein interacts specifically with TLR3, but not with other TLRs. Functional analysis showed that the association of TLR3 and TRIF mediates dsRNA activation of IFNB, through either NFKB, AP1, or IRF3. TRIF activation of NFKB was found to occur predominantly through IRAK1 rather than IRAK2. siRNA blockage of the protein reduced IFNB production in response to dsRNA.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the C-terminus of human TRIF (NNHMWNQRGSQAPEDKTQEAE) was used as the immunogen for this TRIF antibody.
Limitation:
This TRIF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human
Uniprot #:
Q8IUC6