TRIM28 Antibody / KAP1

Product Code: NSJ-R32210

Code	Size	Price

NSJ-R32210-100ug

100 ug

£535.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse
Rat

Applications:

Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

After reconstitution, the TRIM28 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 10

IHC testing of FFPE human breast cancer tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

2 / 10

IHC testing of FFPE human ovarian cancer tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

3 / 10

IHC testing of FFPE human gastric carcinoma tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

4 / 10

IHC testing of FFPE human colorectal adenocarcinoma tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

5 / 10

IHC testing of FFPE human liver cancer tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

6 / 10

IHC testing of FFPE mouse intestinal tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

7 / 10

IHC testing of FFPE rat intestinal tissue with TRIM28 antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to staining.

8 / 10

Western blot testing of 1) human HeLa, 2) human PC-3, 3) human 293T, 4) human A549, 5) rat PC-12, 6) mouse spleen and 7) mouse NIH 3T3 cell lysate with TRIM28 antibody. Observed molecular weight: 88~110 kDa depending on sumoylation.

9 / 10

Flow cytometry testing of human U-2 OS cells with TRIM28 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= TRIM28 antibody.

10 / 10

Immunofluorescent staining of FFPE human ovarian cancer tissue with TRIM28 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.

❮❯

Documents

Further Information

Application Details :

Western blot: 0.1-0.5ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells

Application Note:

Optimal dilution of the TRIM28 antibody should be determined by the researcher.

Description:

Tripartite motif-containing 28, also known as transcriptional intermediary factor 1b (TIF1b) and KAP1 (KRAB-associated protein-1), is a protein that in humans is encoded by the TRIM28 gene. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. KAP1 is a ubiquitously expressed protein involved in many critical functions including: transcriptional regulation, cellular differentiation and proliferation, DNA damage repair, viral suppression, and apoptosis. Its functionality is dependent upon post-translational modifications. Phosphorylation of KAP1 acts as a deactivator of the protein in many of its mechanisms while sumoylation acts as an activator.

Format :

Antigen affinity purified

Formulation :

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Immunogen:

Amino acids 699-835 of human TRIM28 were used as the immunogen for the TRIM28 antibody.

Limitation:

This TRIM28 antibody is available for research use only.

Localization:

Nuclear

Purity:

Antigen affinity

Species Reactivity :

Human, Mouse, Rat

Uniprot #:

Q13263