TRPC3 Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R30846
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30846-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the TRPC3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 2
Western blot testing of TRPC3 antibody and Lane 1: rat liver; 2: (r) lung; 3: (r) intestine; 4: (r) ovary; 5: human U87; 6: (h) A549; 7: (h) COLO320; 8: (h) SW620; 9: (h) HeLa~
2 / 2
IHC-P: TRPC3 antibody testing of rat brain tissue
❮❯

Western blot testing of TRPC3 antibody and Lane 1: rat liver; 2: (r) lung; 3: (r) intestine; 4: (r) ovary; 5: human U87; 6: (h) A549; 7: (h) COLO320; 8: (h) SW620; 9: (h) HeLa~
IHC-P: TRPC3 antibody testing of rat brain tissue

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the TRPC3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Transient receptor potential cation channel, subfamily C, member 3, also known as TRP3, is expressed in megakaryocytic cell lines and therefore may play a role in calcium homeostasis in megakaryocytes and platelets. Using patch-clamp and whole-cell recordings of human embryonic kidney cells transfected with TRPC3, the protein behaved as an inositol phosphate and diacylglycerol-gated channel, but not as a store-operated channel. The TRPC3 gene, but not other TRPC genes, was damaged in several mutant T-cell lines with defective calcium influx, resulting in expression of truncated transcripts. Reduced calcium channel activity could be reversed by restoration of wildtype TRPC3. It is part of endogenous calcium channels in T lymphocytes and contributes to T-cell receptor-dependent calcium entry into T cells. Neuropathologic studies showed impaired growth and differentiation of dendritic arbors of the Purkinje cells, suggesting that normal TRPC3 plays a role in the development and survival of cerebellar Purkinje cells.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 836-851 (HSFNSILNQPTRYQQI) were used as the immunogen for this TRPC3 antibody (100% homologous in human, mouse and rat).
Limitation:
This TRPC3 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13507