Tubulin beta 3 Antibody / TUBB3

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NSJ Bioreagents
Product Code: NSJ-R32611
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32611-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Beta III Tubulin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IHC testing of FFPE human breast cancer with Beta III Tubulin antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC testing of FFPE human glioma with Beta III Tubulin antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
Western blot testing of 1) rat brain, 2) mouse brain and 3) human HeLa lysate with Beta III Tubulin antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.
4 / 5
Flow cytometry testing of human U-2 OS cells with Beta III Tubulin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta III Tubulin antibody.
5 / 5
Flow cytometry testing of human SiHa cells with Beta III Tubulin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta III Tubulin antibody.
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IHC testing of FFPE human breast cancer with Beta III Tubulin antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human glioma with Beta III Tubulin antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat brain, 2) mouse brain and 3) human HeLa lysate with Beta III Tubulin antibody at 0.5ug/ml. Predicted molecular weight ~50 kDa.
Flow cytometry testing of human U-2 OS cells with Beta III Tubulin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta III Tubulin antibody.
Flow cytometry testing of human SiHa cells with Beta III Tubulin antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Beta III Tubulin antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the Beta III Tubulin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Tubulin beta-3 chain is a protein that in humans is encoded by the TUBB3 gene. This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 383-412 (EQFTAMFRRKAFLHWYTGEGMDEMEFTEAE-human) were used as the immunogen for the Beta III Tubulin antibody.
Limitation:
This antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q13509