UBA2 Antibody / SUMO-activating enzyme subunit 2 / SAE2

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NSJ Bioreagents
Product Code: NSJ-RQ4241
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4241-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the UBA2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human 1) HeLa 2) placenta, 3) MCF7, 4) A549, 5) SK-OV-3, 6) 22RV1, 7) A431 and 8) COLO320 lysate with UBA2 antibody at 0.5ug/ml. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.
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Western blot testing of rat 1) brain 2) lung, 3) liver and mouse 4) brain, 5) lung, 6) liver, 7) testis lysate with UBA2 antibody at 0.5ug/ml. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.
3 / 8
IHC testing of FFPE human colon cancer tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
4 / 8
IHC testing of FFPE human breast cancer tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
5 / 8
IHC testing of FFPE mouse testis tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
6 / 8
IHC testing of FFPE rat testis tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
7 / 8
IF/ICC staining of FFPE human A431 cells with UBA2 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
8 / 8
Flow cytometry testing of human A431 cells with UBA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBA2 antibody.
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Western blot testing of human 1) HeLa 2) placenta, 3) MCF7, 4) A549, 5) SK-OV-3, 6) 22RV1, 7) A431 and 8) COLO320 lysate with UBA2 antibody at 0.5ug/ml. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.
Western blot testing of rat 1) brain 2) lung, 3) liver and mouse 4) brain, 5) lung, 6) liver, 7) testis lysate with UBA2 antibody at 0.5ug/ml. Predicted molecular weight: ~72 kDa but routinely observed at ~90 kDa.
IHC testing of FFPE human colon cancer tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human breast cancer tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse testis tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE rat testis tissue with UBA2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IF/ICC staining of FFPE human A431 cells with UBA2 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Flow cytometry testing of human A431 cells with UBA2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= UBA2 antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the UBA2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Ubiquitin-like 1-activating enzyme E1B (UBLE1B) also known as SUMO-activating enzyme subunit 2 (SAE2) is an enzyme that in humans is encoded by the UBA2 gene. Posttranslational modification of proteins by the addition of the small protein SUMO, or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids E449-K564 was used as the immunogen for the SAE2 antibody.
Limitation:
This UBA2 antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9UBT2