VAPB Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4371
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4371-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the VAPB antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 10
Western blot testing of 1) human PANC-1, 2) human SGC-7901, 3) human A549, 4) rat testis and 5) mouse testis lysate with VAPB antibody at 0.5ug/ml. Predicted molecular weight ~27 kDa.
2 / 10
Western blot testing of human 1) MCF-7, 2) HeLa, 3) COLO-320, 4) HepG2, 5) PANC-1, 6) A375, 7) Jurkat and 8) placenta lysate with VAPB antibody at 0.5ug/ml. Predicted molecular weight ~27 kDa.
3 / 10
Western blot testing of mouse 1) liver, 2) heart, 3) skeletal muscle and 4) HEPA1-6 lysate with VAPB antibody at 0.5ug/ml. Predicted molecular weight ~27 kDa.
4 / 10
Immunofluorescent staining of FFPE human A431 cells with VAPB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 10
IHC staining of FFPE human renal cancer with VAPB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human renal cancer with VAPB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human liver cancer with VAPB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE mouse lung with VAPB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 10
Flow cytometry testing of human U-87 MG cells with VAPB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VAPB antibody.
10 / 10
Flow cytometry testing of human Caco-2 cells with VAPB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VAPB antibody.
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Western blot testing of 1) human PANC-1, 2) human SGC-7901, 3) human A549, 4) rat testis and 5) mouse testis lysate with VAPB antibody at 0.5ug/ml. Predicted molecular weight ~27 kDa.
Western blot testing of human 1) MCF-7, 2) HeLa, 3) COLO-320, 4) HepG2, 5) PANC-1, 6) A375, 7) Jurkat and 8) placenta lysate with VAPB antibody at 0.5ug/ml. Predicted molecular weight ~27 kDa.
Western blot testing of mouse 1) liver, 2) heart, 3) skeletal muscle and 4) HEPA1-6 lysate with VAPB antibody at 0.5ug/ml. Predicted molecular weight ~27 kDa.
Immunofluorescent staining of FFPE human A431 cells with VAPB antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE human renal cancer with VAPB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cancer with VAPB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer with VAPB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse lung with VAPB antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Flow cytometry testing of human U-87 MG cells with VAPB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VAPB antibody.
Flow cytometry testing of human Caco-2 cells with VAPB antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VAPB antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the VAPB antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Vesicle-associated membrane protein-associated protein B/C is a protein that in humans is encoded by the VAPB gene. The VAPB gene is found on the 20th human chromosome. The protein encoded by this gene is a type IV membrane protein found in plasma and intracellular vesicle membranes. The encoded protein is found as a homodimer and as a heterodimer with VAPA. This protein also can interact with VAMP1 and VAMP2 and may be involved in vesicle trafficking.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human protein corresponding to amino acids A2-R55 was used as the immunogen for the VAPB antibody.
Limitation:
This VAPB antibody is available for research use only.
Localization:
Cytoplasm (Endoplasmic reticulum membrane)
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
O95292