VASA Antibody (DDX4)

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NSJ Bioreagents
Product Code: NSJ-R31067
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31067-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the VASA antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of VASA antibody and Lane 1: rat testis; 2: mouse testis; 3: HeLa cell lysate
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Western blot testing of VASA antibody and Lane 1: rat testis; 2: mouse testis; 3: (m) ovary tissue lysate
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ICC testing of VASA antibody and MCF-7 cell
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IHC-P testing of rat testis tissue
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IHC-P: VASA antibody testing of rat ovary tissue
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IHC-F testing of rat intestine tissue
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IHC staining of FFPE human ovarian cancer with VISA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Flow cytometry testing of human PC-3 cells with VISA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VISA antibody.
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Flow cytometry testing of human SiHa cells with VISA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VISA antibody.
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Western blot testing of VASA antibody and Lane 1: rat testis; 2: mouse testis; 3: HeLa cell lysate
Western blot testing of VASA antibody and Lane 1: rat testis; 2: mouse testis; 3: (m) ovary tissue lysate
ICC testing of VASA antibody and MCF-7 cell
IHC-P testing of rat testis tissue
IHC-P: VASA antibody testing of rat ovary tissue
IHC-F testing of rat intestine tissue
IHC staining of FFPE human ovarian cancer with VISA antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Flow cytometry testing of human PC-3 cells with VISA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VISA antibody.
Flow cytometry testing of human SiHa cells with VISA antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VISA antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the VASA antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
DEAD/H BOX 4, also known as DDX4 and VASA contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse homolog resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 253-272 (EDEDSIFAHYQTGINFDKYD) were used as the immunogen for this VASA antibody (100% human, mouse and rat homology).
Limitation:
This VASA antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q9NQI0