VASP Antibody

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NSJ Bioreagents
Product Code: NSJ-R32632
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32632-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the VASP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IHC testing of FFPE human lung cancer tissue with VASP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
2 / 5
IHC testing of FFPE human breast cancer tissue with VASP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
3 / 5
Western blot testing of 1) rat liver, 2) mouse kidney, 3) human HeLa and 4) human HePG2 lysate with VASP antibody at 0.5ug/ml. Expected molecular weight: ~40/46-50 kDa (unmodified/phosphorylated).
4 / 5
Flow cytometry testing of human SiHa cells with VASP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VASP antibody.
5 / 5
Flow cytometry testing of human U937 cells with VASP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VASP antibody.
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IHC testing of FFPE human lung cancer tissue with VASP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
IHC testing of FFPE human breast cancer tissue with VASP antibody at 1ug/ml. HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of 1) rat liver, 2) mouse kidney, 3) human HeLa and 4) human HePG2 lysate with VASP antibody at 0.5ug/ml. Expected molecular weight: ~40/46-50 kDa (unmodified/phosphorylated).
Flow cytometry testing of human SiHa cells with VASP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VASP antibody.
Flow cytometry testing of human U937 cells with VASP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VASP antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the VASP antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
Description:
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids 78-114 (NFHQWRDARQVWGLNFGSKEDAAQFAAGMASALEALE-human) were used as the immunogen for this VASP antibody.
Limitation:
This antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P50552