VE cadherin Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4336
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4336-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the VE cadherin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of 1) mouse lung, 2) mouse liver and 3) rat PC-12 lysate with VE cadherin antibody at 0.5ug/ml. Expected molecular weight: 90~140 kDa depending on glycosylation level.
2 / 2
IHC testing of FFPE mouse kidney tissue with VE cadherin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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Western blot testing of 1) mouse lung, 2) mouse liver and 3) rat PC-12 lysate with VE cadherin antibody at 0.5ug/ml. Expected molecular weight: 90~140 kDa depending on glycosylation level.
IHC testing of FFPE mouse kidney tissue with VE cadherin antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA (Capture; mouse recombinant protein): 1-5ug/ml
Application Note:
Optimal dilution of the VE cadherin antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse protein corresponding to amino acids D46-E285 was used as the immunogen for the VE cadherin antibody.
Limitation:
This VE cadherin antibody is available for research use only.
Localization:
Cell junction
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P55284