VE Cadherin Antibody / CDH5 (Intracellular Junction Marker)

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NSJ Bioreagents
Product Code: NSJ-RQ4111
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4111-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the CDH5 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of human placenta lysate with CDH5 antibody at 0.5ug/ml. Predicted molecular weight: 90~140 kDa depending on glycosylation level.
2 / 10
Western blot testing of 1) human A431, 2) rat liver and 3) rat kidney lysate with CDH5 antibody at 0.5ug/ml. Expected molecular weight: 90~140 kDa depending on glycosylation level.
3 / 10
IF/ICC staining of FFPE human A549 cells with CDH5 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
4 / 10
Flow cytometry testing of human A431 cells with CDH5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDH5 antibody.
5 / 10
IHC staining of FFPE human lung cancer with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
6 / 10
IHC staining of FFPE human breast cancer with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
7 / 10
IHC staining of FFPE human breast cancer with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
8 / 10
IHC staining of FFPE human rectal cancer with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
9 / 10
IHC staining of frozen human placenta with CDH5 antibody.
10 / 10
IHC staining of FFPE mouse lung with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
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Western blot testing of human placenta lysate with CDH5 antibody at 0.5ug/ml. Predicted molecular weight: 90~140 kDa depending on glycosylation level.
Western blot testing of 1) human A431, 2) rat liver and 3) rat kidney lysate with CDH5 antibody at 0.5ug/ml. Expected molecular weight: 90~140 kDa depending on glycosylation level.
IF/ICC staining of FFPE human A549 cells with CDH5 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A431 cells with CDH5 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= CDH5 antibody.
IHC staining of FFPE human lung cancer with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human breast cancer with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of frozen human placenta with CDH5 antibody.
IHC staining of FFPE mouse lung with CDH5 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml,Flow cytometry: 1-3ug/million cells,Immunofluorescence/Immunocytochemistry: 4-6ug/ml,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the CDH5 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant human partial protein corresponding to amino acids D48-R272 was used as the immunogen for the CDH5 antibody.
Limitation:
This CDH5 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P33151