VEGF Receptor 1 / VEGFR1 Antibody

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NSJ Bioreagents
Product Code: NSJ-RQ4032
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4032-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Storage:
After reconstitution, the VEGFR1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Immunofluorescent staining of FFPE human A549 cells with VEGFR1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Flow cytometry testing of human A549 cells with VEGFR1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VEGFR1 antibody.
3 / 3
Western blot testing of human 1) HeLa, 2) placenta, 3) A549, 4) COLO320, 5) A431 and 6) SKOV3 cell lysate with VEGFR1 antibody at 0.5ug/ml. Predicted molecular weight ~150 kDa.
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Immunofluorescent staining of FFPE human A549 cells with VEGFR1 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human A549 cells with VEGFR1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VEGFR1 antibody.
Western blot testing of human 1) HeLa, 2) placenta, 3) A549, 4) COLO320, 5) A431 and 6) SKOV3 cell lysate with VEGFR1 antibody at 0.5ug/ml. Predicted molecular weight ~150 kDa.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunofluorescence (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells,ELISA (Capture): 0.1-0.5ug/ml
Application Note:
Optimal dilution of the VEGFR1 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
Vascular endothelial growth factor receptor 1 (FLT1) is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family. It is mapped to 13q12. The deduced 1,338-amino acid protein has a calculated molecular mass of 150.6 kD. It has a 758-amino acid extracellular domain, followed by a 22-amino acid transmembrane region and a 558-amino acid cytoplasmic region containing a cluster of basic amino acids and a tyrosine kinase domain. sFLT-1 was identified in placenta, adult lung, kidney, liver and uterus. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids AVKMLKEGATASEYKALMTELKILTHIGHHLNVVNLL from the human protein were used as the immunogen for the VEGFR1 antibody.
Limitation:
This VEGFR1 antibody is available for research use only.
Purity:
Antigen affinity purified
Species Reactivity :
Human
Uniprot #:
P17948