Vegf receptor 2 Antibody / Vegfr2 / Kdr

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NSJ Bioreagents
Product Code: NSJ-RQ4055
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-RQ4055-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Vegf receptor 2 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IHC testing of FFPE rat kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
2 / 5
IHC testing of FFPE mouse kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 5
Western blot testing of recombinant mouse Vegfr2 protein with Vegf receptor 2 antibody at 0.5ug/ml.
4 / 5
Western blot testing of mouse 1) heart, 2) thymus, 3) spleen, 4) kidney, 5) brain, 6) lung, 7) HEPA1-6 and 8) NIH 3T3 lysate with Vegf receptor 2 antibody. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).
5 / 5
Immunofluorescent staining of FFPE human NIH 3T3 cells with Vegf receptor 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
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IHC testing of FFPE rat kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse kidney tissue with Vegf receptor 2 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Western blot testing of recombinant mouse Vegfr2 protein with Vegf receptor 2 antibody at 0.5ug/ml.
Western blot testing of mouse 1) heart, 2) thymus, 3) spleen, 4) kidney, 5) brain, 6) lung, 7) HEPA1-6 and 8) NIH 3T3 lysate with Vegf receptor 2 antibody. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).
Immunofluorescent staining of FFPE human NIH 3T3 cells with Vegf receptor 2 antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,ELISA (Capture): 0.1-0.5ug/ml,Immunofluorescence (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the Vegf receptor 2 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
Description:
KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Vascular endothelial growth factor (VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1 (Flk1) in mouse, is exclusively expressed in endothelial cells. Flk1 is the mouse homolog of KDR.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
A recombinant mouse partial protein corresponding to amino acids A20-L244 was used as the immunogen for the Vegf receptor 2 antibody.
Limitation:
This Vegf receptor 2 antibody is available for research use only.
Localization:
Cell junction, cytoplasmic
Purity:
Antigen affinity purified
Species Reactivity :
Mouse, Rat
Uniprot #:
P35918