VEGF Receptor 3 Antibody / VEGFR3

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NSJ Bioreagents
Product Code: NSJ-R32892
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32892-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the VEGFR3 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 10
Western blot testing of human 1) HeLa, 2) MCF7 and 3) HepG2 cell lysate with VEGFR3 antibody at 0.5ug/ml. Predicted molecular weight ~153 kDa (long), ~147 kDa (short) and ~93 kDa (sVegfr3).
2 / 10
IHC testing of FFPE human lung cancer tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
3 / 10
IHC testing of FFPE human prostate cancer tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
4 / 10
IHC testing of FFPE human breast cancer tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
5 / 10
IHC testing of FFPE human colon cancer tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
6 / 10
IHC testing of FFPE human placental tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
7 / 10
IHC testing of FFPE mouse liver tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
8 / 10
IHC testing of FFPE rat liver tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
9 / 10
IF/ICC staining of FFPE human A431 cells with VEGFR3 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
10 / 10
Flow cytometry testing of human U-2 OS cells with VEGFR3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VEGFR3 antibody.
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Western blot testing of human 1) HeLa, 2) MCF7 and 3) HepG2 cell lysate with VEGFR3 antibody at 0.5ug/ml. Predicted molecular weight ~153 kDa (long), ~147 kDa (short) and ~93 kDa (sVegfr3).
IHC testing of FFPE human lung cancer tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human prostate cancer tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human breast cancer tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human colon cancer tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE human placental tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE mouse liver tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IHC testing of FFPE rat liver tissue with VEGFR3 antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
IF/ICC staining of FFPE human A431 cells with VEGFR3 antibody (green) at 2ug/ml and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human U-2 OS cells with VEGFR3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VEGFR3 antibody.

Documents

Further Information

Application Details :
Western Blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Immunofluorescence/Immunocytochemistry (FFPE): 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
Optimal dilution of the VEGFR3 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
Description:
Fms-related tyrosine kinase 4, also known as FLT4 or VEGFR3, is a protein which in humans is encoded by the FLT4 gene. It is mapped to 5q35.3. This gene encodes a tyrosine kinase receptor for vascular endothelial growth factors C and D. The protein is thought to be involved in lymphangiogenesis and maintenance of the lymphatic endothelium. FLT4 has an essential role in the development of the embryonic cardiovascular system before the emergence of the lymphatic vessels. It has been found that FLT4, which provides proangiogenic signaling when expressed on endothelium, may also have antiangiogenic properties when expressed at an avascular site by nonendothelial cells. FLT4 is also regarded as a regulator of vascular network formation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids SCRGQHPLEWAWPGAQEAPATGDKDSEDT were used as the immunogen for the VEGFR3 antibody.
Limitation:
This VEGFR3 antibody is available for research use only.
Localization:
Cytoplasmic, nuclear, cell membrane
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P35916