VEGFA Antibody

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NSJ Bioreagents
Product Code: NSJ-R31500
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31500-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the VEGF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 5
IHC staining of FFPE human rectal cancer with VEGF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
2 / 5
IHC staining of FFPE human rectal cancer with VEGF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
3 / 5
IHC staining of FFPE rat brain with VEGF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
4 / 5
Western blot testing of 1) rat lung, 2) rat liver, 3) rat thymus, 4) mouse lung, 5) mouse thymus, 6) mouse HEPA1-6, 7) human placenta and 8) human A431 lysate with VEGF antibody. Predicted molecular weight 19~22 kDa (monomer) and 38~44 kDa (dimer).
5 / 5
Flow cytometry testing of human SiHa cells with VEGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VEGF antibody.
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IHC staining of FFPE human rectal cancer with VEGF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer with VEGF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
IHC staining of FFPE rat brain with VEGF antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Western blot testing of 1) rat lung, 2) rat liver, 3) rat thymus, 4) mouse lung, 5) mouse thymus, 6) mouse HEPA1-6, 7) human placenta and 8) human A431 lysate with VEGF antibody. Predicted molecular weight 19~22 kDa (monomer) and 38~44 kDa (dimer).
Flow cytometry testing of human SiHa cells with VEGF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VEGF antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting points. Titration of the VEGF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
VEGF is the only mitogen that specifically acts on endothelial cells. It may be a major regulator of tumor angiogenesis in vivo. It is structurally related to platelet-derived growth factor. VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB. VEGF gene contains 8 exons. VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung. And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism. What?s more, it also stimulates neurogenesis in vitro and in vivo.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
7422
Immunogen:
Human recombinant protein, A isoform (AA 27-191) was used as the immunogen for this VEGF antibody.
Limitation:
This VEGF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat