Vimentin Antibody

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NSJ Bioreagents
Product Code: NSJ-R30090
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30090-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: V9, aka VMT-24
Regulatory Status: RUO
Target Species:
  • Human
  • Porcine (pig)
  • Rabbit
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the Vimentin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 11
Immunofluorescent staining of FFPE human intestinal cancer with Vimentin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
2 / 11
IHC staining of FFPE human invasive urothelial carcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 11
IHC staining of FFPE human invasive urothelial carcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 11
IHC staining of FFPE human lung adenocarcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 11
IHC staining of FFPE human colorectal adenocarcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
6 / 11
IHC staining of FFPE human liver cancer tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
7 / 11
IHC staining of FFPE human tonsil tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
8 / 11
IHC staining of FFPE human placental tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
9 / 11
IHC-P: Vimentin antibody testing of human breast cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
10 / 11
IHC staining of FFPE rat brain tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
11 / 11
Western blot testing of human 1) HeLa, 2) A375, 3) U-87 MG and 4) HepG2 cell lysate with Vimentin antibody. Predicted molecular weight ~54 kDa.
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Immunofluorescent staining of FFPE human intestinal cancer with Vimentin antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA for 20 min.
IHC staining of FFPE human invasive urothelial carcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human invasive urothelial carcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human lung adenocarcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human colorectal adenocarcinoma tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human liver cancer tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human placental tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC-P: Vimentin antibody testing of human breast cancer tissue. HIER: steam section in pH6 citrate buffer for 20 min.
IHC staining of FFPE rat brain tissue with Vimentin antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) HeLa, 2) A375, 3) U-87 MG and 4) HepG2 cell lysate with Vimentin antibody. Predicted molecular weight ~54 kDa.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1.5-2.5ug/ml,Immunofluorescence: 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Vimentin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
The VIM gene was one of many that Gieser and Swaroop (1992) recovered from a subtracted cDNA library for retinal pigment epithelium. The Vimentin gene expresses in human lymphocytes and in Burkitts lymphoma cells and it is secreted by activated macrophages. The gene encoding human protein is located on the short arm of chromosome 10.
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
7431
Immunogen:
Pig eye lens vimentin was used as the immunogen for this Vimentin antibody.
Limitation:
This Vimentin antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, rabbit, pig, rat