Vinculin Antibody

Contact us
NSJ Bioreagents
Product Code: NSJ-R30091
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30091-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Mouse
Antibody Isotype: Mouse IgG1
Antibody Clonality: Monoclonal
Antibody Clone: HVIN-1, aka VIN-54
Regulatory Status: RUO
Target Species:
  • Chicken (Gallus)
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Western Blot (WB)
Storage:
After reconstitution, the Vinculin antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey COS-7, 4) human U-87 MG, 5) rat PC-12, 6) rat RH35, 7) mouse HEPA1-6, and 8) mouse NIH3T3 lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
2 / 4
IHC testing of frozen human placenta tissue with Vinculin antibody.
3 / 4
IHC testing of frozen human placenta tissue with Vinculin antibody.
4 / 4
Immunofluorescent staining of FFPE human breast cancer with Vinculin antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
❮❯

Western blot testing of 1) human HeLa, 2) human HepG2, 3) monkey COS-7, 4) human U-87 MG, 5) rat PC-12, 6) rat RH35, 7) mouse HEPA1-6, and 8) mouse NIH3T3 lysate with Vinculin antibody. Predicted molecular weight ~124 kDa.
IHC testing of frozen human placenta tissue with Vinculin antibody.
IHC testing of frozen human placenta tissue with Vinculin antibody.
Immunofluorescent staining of FFPE human breast cancer with Vinculin antibody (green) and DAPI (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.

Documents

Further Information

Application Details :
Western blot: 1-2ug/ml,Immunohistochemistry (Frozen): 2-4ug/ml for formalin or acetone fixed tissues,Immunofluorescence: 2-4ug/ml
Application Note:
The stated application concentrations are suggested starting points. Titration of the Vinculin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Vinculin is a cytoskeletal protein associated with the cytoplasmic face of both cell-cell and cell-extracellular matrix adherens-type junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Both human and chicken embryo sequences of vinculin contain 1,066 amino acids and the two proteins exhibit a high level of sequence identity (greater than 95%).
Format :
Ascites
Formulation :
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Gene ID #:
7414
Immunogen:
Human vinculin purified from uterus was used as the immunogen for this Vinculin antibody.
Limitation:
This Vinculin antibody is available for research use only.
Purity:
Unpurified ascites
Species Reactivity :
Human, mouse, rat, chicken