VIP Antibody / PHM-27

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NSJ Bioreagents
Product Code: NSJ-R32397
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32397-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
After reconstitution, the VIP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC testing of FFPE human pancreas with VIP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
2 / 6
IHC testing of FFPE mouse pancreas with VIP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
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IHC testing of FFPE rat pancreas with VIP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
4 / 6
IHC testing of FFPE mouse intestinal tissue with VIP antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
5 / 6
IHC testing of FFPE rat intestinal tissue with VIP antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
6 / 6
Immunofluorescent staining of FFPE rat colon tissue with VIP antibody (red) and DAPI nuclear stain (blue). HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
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IHC testing of FFPE human pancreas with VIP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse pancreas with VIP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat pancreas with VIP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE mouse intestinal tissue with VIP antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
IHC testing of FFPE rat intestinal tissue with VIP antibody. HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.
Immunofluorescent staining of FFPE rat colon tissue with VIP antibody (red) and DAPI nuclear stain (blue). HIER: Boil the paraffin sections in pH8 EDTA buffer for 20 minutes and allow to cool prior to testing.

Documents

Further Information

Application Details :
IHC (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the VIP antibody should be determined by the researcher.
Description:
Vasoactive intestinal peptide, also known as PHM27 or VIP, is a peptide hormone containing 28 amino acid residues. This gene is mapped to 6q25. The protein encoded by this gene belongs to the glucagon family. It stimulates myocardial contractility, causes vasodilation, increases glycogenolysis, lowers arterial blood pressure and relaxes the smooth muscle of trachea, stomach and gall bladder. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids HADGVFTSDFSKLLGQLSAKKYLESLM (PHM-27 sequence) from the human protein were used as the immunogen for the VIP antibody.
Limitation:
This VIP antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P01282