VWF Antibody

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NSJ Bioreagents
Product Code: NSJ-R31491
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R31491-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the VWF antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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Western blot testing of VWF antibody and HT1080 lysate. Expected/observed size ~309KD
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IHC-P: VWF antibody testing of human lung cancer tissue
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IHC testing of frozen human placental tissue with VWF antibody.
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Flow cytometry testing of human A431 cells with VWF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VWF antibody.
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Western blot testing of VWF antibody and HT1080 lysate. Expected/observed size ~309KD
IHC-P: VWF antibody testing of human lung cancer tissue
IHC testing of frozen human placental tissue with VWF antibody.
Flow cytometry testing of human A431 cells with VWF antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= VWF antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the VWF antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The gene encodes a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
7450
Immunogen:
Human partial recombinant protein (AA 2535-2813) was used as the immunogen for this VWF antibody.
Limitation:
This VWF antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human