WASP Antibody

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NSJ Bioreagents
Product Code: NSJ-R32099
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R32099-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunocytochemistry (ICC)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
After reconstitution, the WASP antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

1 / 4
Western blot testing of 1) rat liver, 2) human placenta, 3) rat spleen, 4) rat pancreas, 5) human HepG2, 6) HeLa, 7) HEPA and 8) 22RV1 lysate with WASP antibody. Expected/observed molecular weight ~53 kDa.
2 / 4
IHC testing of FFPE human lung cancer tissue with WASP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
3 / 4
IHC testing of frozen human placenta with WASP antibody.
4 / 4
ICC testing of A549 cells with WASP antibody.
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Western blot testing of 1) rat liver, 2) human placenta, 3) rat spleen, 4) rat pancreas, 5) human HepG2, 6) HeLa, 7) HEPA and 8) 22RV1 lysate with WASP antibody. Expected/observed molecular weight ~53 kDa.
IHC testing of FFPE human lung cancer tissue with WASP antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
IHC testing of frozen human placenta with WASP antibody.
ICC testing of A549 cells with WASP antibody.

Documents

Further Information

Application Details :
Western blot: 0.1-0.5ug/ml,Immunocytochemistry: 0.5-1ug/ml,Immunohistochemistry (Frozen): 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml
Application Note:
Optimal dilution of the WASP antibody should be determined by the researcher.
Description:
The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. Wiskott-Aldrich syndrome is a rare, inherited, X-linked, recessive disease characterized by immune dysregulation and microthrombocytopenia, and is caused by mutations in the WAS gene. The WAS gene product is a cytoplasmic protein, expressed exclusively in hematopoietic cells, which show signalling and cytoskeletal abnormalities in WAS patients. A transcript variant arising as a result of alternative promoter usage, and containing a different 5' UTR sequence, has been described, however, its full-length nature is not known.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids ADEDEAQAFRALVQEKIQKRNQRQSGDR of human WASP were used as the immunogen for the WASP antibody.
Limitation:
This WASP antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P42768