YB1 Antibody

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NSJ Bioreagents
Product Code: NSJ-R30851
Product Group: Primary Antibodies
Supplier: NSJ Bioreagents
CodeSizePrice
NSJ-R30851-100ug100 ug£535.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application: Western Blot (WB)
Storage:
After reconstitution, the YB1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.

Images

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IHC staining of FFPE human ovarian cancer tissue with YB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human rectal cancer tissue with YB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 6
IHC staining of FFPE mouse testis tissue with YB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
4 / 6
IHC staining of FFPE rat testis tissue with YB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
5 / 6
Western blot testing of 1) human MCF7, 2) human 293T, 3) human HeLa, 4) human Jurkat, 5) human T-47D, 6) human ThP-1, 7) human MOLT-4, 8) human HL60, 9) rat testis, 10) mouse stomach and 11) mouse testis tissue lysate with YB1 antibody. Expected molecular weight: 39-50 kDa depending on glycosylation level.
6 / 6
Flow cytometry testing of human HEL cells with YB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= YB1 antibody.
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IHC staining of FFPE human ovarian cancer tissue with YB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human rectal cancer tissue with YB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE mouse testis tissue with YB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat testis tissue with YB1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) human MCF7, 2) human 293T, 3) human HeLa, 4) human Jurkat, 5) human T-47D, 6) human ThP-1, 7) human MOLT-4, 8) human HL60, 9) rat testis, 10) mouse stomach and 11) mouse testis tissue lysate with YB1 antibody. Expected molecular weight: 39-50 kDa depending on glycosylation level.
Flow cytometry testing of human HEL cells with YB1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= YB1 antibody.

Documents

Further Information

Application Details :
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the YB1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Y box binding protein 1, commonly referred to as YBX1 or just YB1 by researchers, is a human protein. YBX1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box. It has a molecular mass of 35,414D and contains 18% basic residues and putative nuclear localization signals. The protein is highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but is downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation. YBX1 is expressed at high levels in myeloid leukemic cells at different developmental stages. Knockdown of the protein in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment. YBX1 is downregulated during myeloid differentiation and aberrant expression in leukemic cells may contribute to leukemia development by blocking differentiation.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
An amino acid sequence from the middle region of human YBX1 (KYAADRNHYRRYPRRR) was used as the immunogen for this YB1 antibody (100% homologous in human, mouse and rat).
Limitation:
This YB1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
P67809